. 40266 Email: drugdiscoverytech@lifetech. 7. +1 800-635-5577. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. . This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Table of Contents. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. ® reader to the computer. Exhibit with us {{>currencyLabel}} Back. 4. . The plate reader can measure multiple readouts, including UV and visible absorbance. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. View the Molecular Devices website. Examples: “Press carefully to engage,” “ Do not press down. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The dual monochromator optics allow the widest range of applications to be utilized for bior. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Methods Preparation of reagents and standards. Description 1. 95. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. The M5 can read volumes down to 2uL with the. Plate chamber design of the SpectraMax category 1 microplate readers. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. SpectraMax Spectrofluorometers. Spectral Range. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The chamber. The viscosity solution was prepared by mixing PBS and Glycerol in different proportion. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Lower limits of detection for ATP determined using the ATPlite . Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Wash Buffer Concentrate was diluted 10-fold. 0 mL). 4. Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . 2. There were 12 replicate wells per dilution in both 96-well and 384- well plates. . The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Figure 1. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. The SpectraMax M2 and M2e are versatile and reliable. The guide also covers the software features, the specifications, and the accessories of the systems. Molecular Devices. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. The dual monochromator optics allow. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. For example, microplates containing different samp les can be read using the same or Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Other technologies such as imaging. , ~488 nm), has the optical. 01) + 0. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). e. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. the IMAP Platform and smoothly transition into high-throughput screening. 2. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. In either case. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 19. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 0 (IBM Corporation, Armonk, NY, USA). The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Wavelength Range. 1step assay with SpectraMax microplate readers. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Last lab had ours for years and never had a problem with it. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Figure 2. We would like to show you a description here but the site won’t allow us. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts. Step 4: Incubate at room temperature for 1 hour, protected from light. Description 1. The analysis capabilities ofThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. All reagents were brought to room temperature before use. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. Fluorescence intensity was measured using a. Step 1. Open the Softmax Pro software and select the FP option. . 3: The cuvette chamber. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Features. 0 out of 5 $ 17,999 $ 16,995. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 3: The cuvette chamber. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. The following document is intended to demonstrate setup of this instrument. SpectraMax Glo and DuoLuc. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. 0 out of 5 $ 15,999. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. The M5 has two PMTs. com A. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. We would like to show you a description here but the site won’t allow us. Maximum signal with lowest background. Published Dec 16, 2020 | Updated Dec 16, 2020. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. When the correct COM port is selected, the. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Flexible for 6-well to 384-well microplates. 7 nM EGF, the EC 80 concentration determined from. . Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. type Clear. Wavelength Range. Note: In this user guide, all references to SpectraMax Multi-Mode. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 6. $4,499. Here is a Molecular Devices Spectramax M5 Microplate Reader for sale. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 315 can't find zero order. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Pick the best speed, sensitivity, and price for your needs. . The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. . Multimode Reader Comparison Chart. 2. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Description 1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Select the fluorescence option and read the total fluorescence. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. $1,250. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. SpectraMax M3, M4, and M5/M5e readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. ISM Equipment Classification (Group 1, Class A) This equipment is. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. 7°C and the bottom to 36. . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax ABS Plus SpectraTest ABS2 v1. Wavelength optimization. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. D 1 1. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. 2. clicked with the SpectraMax L Microplate Reader. . HTRF-compatible SpectraMax Multi-Mode Readers. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. For example, microplates containing different samp les can be read using the same orProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Optimized for fluorescence microplate readers. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. . SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Spectral Range. Microplate Reader Shelf. 3. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. . Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Offer Ends In: 18 Days 19: 38: 21. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Samples were measured together with a corresponding reference solvent contained in a. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 3. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. 1. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. We would like to show you a description here but the site won’t allow us. 1. edu) to be added as a trained user in iLab. 4. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. 4. 3. While they play an important role in generating data from a wide range of. The corrected absorbance is within 5% of the. 72 MB. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Description 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Wide range of applications. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. These robust readers have been placed in labs from Antarctica to the. Step 2. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Highly selective for dsDNA over ssDNA or RNA. 9000-0756. 1. The PathCheck sensor can extend the instrument linearity by a factor of three or more. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. EarlyTox Live Cell Assay (6. . spr SpectraMax iD3 SpectraTest ABS2 v1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Download PDF versions of the SpectraMax M5 User Guides from this page. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 190-1000 nm, tunable in 1 nm increments. Click SoftMax Pro Software User Guide. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Description. 190-1000. UV/Vis Photometric Performance:. Find regional support. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 40266 Email: [email protected]® M5多功能读板机为使用者提 供了除化学发光以外的其他检测方法，例 如：光吸收，荧光强度，荧光偏振和时间 分辨荧光。 SpectraMax® L和SpectraMax® M5酶标 仪均用SoftMax® Pro软件进行操控和设 置，该软件含有CellTiter-Glo检测模板和0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. The SpectraMax ABS Plus reader utilizes Molecular . S. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. e. edu) to be added as a trained user in iLab. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. ORDER. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. 305 reference level saturation. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. Note: In this user guide, SpectraMax ® refers to several SpectraMax . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The REF button has no valid meaning for. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Select a rugged, lightweight model designed to be used in the field. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 1. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. 72 MB. Bottom-reading is usually recommended for cell-based assay with adherent cells. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. Open the Softmax Pro software and select the FP option. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The following document is intended to demonstrate setup of this. For example, microplates containing different samples can be read using the312 gain calibration failed. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The assay window spanned four orders of magnitude. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 314 low lamp level warning. 4. Description. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. The dynamic range of the assay was approximately 5 orders of magnitude. SpectraMax Injector Cartridge with SmartInject™ Technology. Supplier: Molecular Devices. Dual monochromators provide the ability for. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. The. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. 7" color, capacitive touch screen. Softmax Pro GxP Software 6. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. These all-inclusive assay kits are ready to use in assay validation or screening. acquired on a SpectraMax M5 (Molecular Devices). Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. When the user applies a set temperature of 37°C, the top is heated to 37. It does not work as expected. There were 12 replicate wells per dilution in both 96-well and 384- well plates. 2. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The plate reader can measure multiple readouts, including UV. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. No filter change with dual monochromator optics. SoftMax Pro. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Introduction. 304 signal level saturation. SOFTmax PRO UserÕs Manual iii Contents Figures . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. 0 out of 5 $ 15,999. 2. M5. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. SpectraMax M5e Multi-Mode Microplate Reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. These lab workhorses are used in drug discovery, research, bioassay validation, and. 00 USD. D 1 1. Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 0 mm wide, and heights from 14 mm to 15 mm. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. +44-118-944-8000. 3. Add to Cart. Softmax Pro GxP Software 6. TR-FRET assay principle. SpectraMax M2 and SpectraMax M2e are controlled by an external computer running SoftMax Pro software which provides integrated instrument control, data display, andQualify the fluorescence performance of the SpectraMax . Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. . In the M2, M5 and Plus 384, the light. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. View the Molecular Devices website. The M5 can read volumes down to 2uL with the uMax Low Volume. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. . 1. 304 signal level saturation. Sale. spr * SpectraMax i3(x) SpectraTest ABS2 v1. These all-inclusive assay kits are ready to use in assay validation or screening. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 4. NORTH AMERICA. The Spectr. Consistent results are obtained over time offering workflow flexibility. The following document is. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Three kits covering a wide range of dsDNA concentrations. free tracer decreases, resulting in an.